منابع مشابه
Structural studies on the Escherichia coli O101 lipopolysaccharide found in association with F41 and K99 fimbriae.
In this study it was shown that the O101 lipopolysaccharide isolated from Escherichia coli B41 did not contain an O-specific polysaccharide and that its sugar moiety is probably restricted to the core oligosaccharide. It is characterized by the presence of galactose, glucose, N-acetylglucosamine, heptose and 3-deoxy-D-manno-2-octulosonic acid and the fatty acid composition is typical of an Ente...
متن کاملCharacterization of the F41 fimbrial antigen of enterotoxigenic Escherichia coli by using monoclonal antibodies.
We produced monoclonal antibodies (MAbs) from 23 different murine hybridoma cell lines against the F41 fimbrial antigen of bovine and porcine enterotoxigenic Escherichia coli. Cell lines were created by fusing myeloma cells and spleen cells of mice that were immunized with either purified F41 or with Formalin-killed whole cells. The specificity of the MAbs to the F41 antigen was proven by enzym...
متن کاملNucleotide sequence of the F41 fimbriae subunit gene in Escherichia coli B41.
A clone encoding the F41 f imbnal sub-unit gene from E coli B41, was isolated from an B1BL3 library using an oligonucleotide based on the published N-termlnal amino acid sequence (1). The DNA sequence of an t I66bp fragment revealed an open reading frame encoding a protein of 277 amino acids including a 22 amino acid leader sequence (dash underlined). This corresponds to a mature protein of 290...
متن کاملInduction of immune responses in mice after oral immunization with recombinant Lactobacillus casei strains expressing enterotoxigenic Escherichia coli F41 fimbrial protein.
In an effort to develop a safe and effective vaccine for the prevention of enterotoxigenic Escherichia coli (ETEC) F41 infections, we have developed a surface antigen display system using poly-gamma-glutamate synthetase A (PgsA) as an anchoring matrix. The recombinant fusion proteins comprised of PgsA and fimbrial protein of F41 were stably expressed in Lactobacillus casei 525. Surface localiza...
متن کاملPassive immunity in cattle against enterotoxigenic Escherichia coli: serologic evaluation of a bacterin containing K99 and F41 fimbriae in colostrum of vaccinated females and calf serum
A bacterin from enterotoxigenic Escherichia coli (ETEC), containing fimbriae K99 and F41, was produced and its capacity to induce anti-K99 and anti-F41 antibodies in colostrum of vaccinated cows and in calf serum, and the persistence of these antibodies in neonates were determined. Three experiments were performed on two commercial farms. In all experiments animals were allotted randomly to the...
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ژورنال
عنوان ژورنال: Arthropod Management Tests
سال: 2012
ISSN: 2155-9856
DOI: 10.4182/amt.2012.f41